A 5-0 win over Wigan Athletic on Boxing Day saw Jonny Evans forced off with a calf injury and he will now miss the home game with Blackburn Rovers on New Year's Eve.
United had already been forced to field Antonio Valencia and Michael Carrick in defence, with Nemanja Vidic heading a long list of absentees, and will hope that Rio Ferdinand, Phil Jones and Chris Smalling can all overcome problems to be available on Saturday.
Liverpool are deprived of Luis Suarez for Friday night's game against Newcastle United after he was hit with a one-game ban for making a crude gesture at Fulham fans.
That means non-scoring Geordie Andy Carroll will have the chance to stake a claim for a regular first-team place when lining up against his former side.
Liverpool skipper Steven Gerrard returned to action from the bench in the 1-1 draw with Blackburn but it remains uncertain whether he will be back in the starting line-up to face Newcastle.
Wolves are also forced to contend with a suspension after their failed appeal against a red card shown to midfielder Nenad Milijas in the 1-1 draw with Arsenal - he must serve a three-game suspension.
However, Wigan's appeal against the dismissal of Conor Sammon against Manchester United has been successful and he is free to face Stoke City.