Ware landed awkwardly while trying to block a shot in the first half and his right leg bent at a gruesome angle, leaving the crowd at Lucas Oil Stadium in Indianapolis in a stunned silence.
After a nine-minute delay, Ware was carted off the court on a stretcher leaving Louisville players and coach Rick Pitino in tears.
The Louisville team was able to recover for an 85-63 victory over Duke that advanced them to the Final Four but their thoughts remained with their team mate.
Close friend and team mate Chane Behanan changed into Ware's number five jersey after speaking with him in the locker room.
Ware was taken to a nearby hospital and underwent surgery but there was no timetable placed on his recovery.