Frenchman Fourcade headed to Oslo looking to get back to winning ways after claiming just one gold but three silver medals from the Worlds.
But it was a case of so close but still so far for the 24-year-old as he was once again second best in the men's 10km sprint, despite putting in the three fastest laps.
It was two missed targets that let Fourcade down as he had to settle for second by just 0.1 seconds.
That was because Norwegian Boe was perfect with the rifle to take victory in 25:44.5, with Andriy Deryzemlya of Ukraine finishing third.
Despite finishing runner-up yet again Fourcade still looks on course to defend his overall World Cup total as he extended his lead at the top of the standings.
With nearest challenger Emil Hegle Svendssen not in action in Osle Fourcade is now 147 points ahead with just seven events remaining.
Great Britain's Lee-Steve Jackson came home in 60th position, 3:00.7 minutes behind Boe.