The 28-year-old Slovakian, who won Olympic gold in the 7.5km sprint at Vancouver 2010, dominated the same event as the sixth World Cup of the season got under way in Antholz-Anterselva.
Starting in 32nd, Kuzmina took the lead for the first time after her first trip to the range before shooting clear to take victory by over 16 seconds in a winning time of 20:28.6minutes.
Finland's Kaisa Makarainen finished second, 16.7seconds behind Kuzmina, who also took Olympic pursuit silver at Vancouver 2010, with Darya Domracheva of Belarus taking third.
That is Kuzmina's clear best finish of the season with her previous best an eighth in the 10km pursuit in Oestersund while she was ninth last time out in the 12.5km mass start in Ruhpolding.
In stark contrast World Cup leader Berger finished down in 14th for her second worst finish of the season after missing three targets and coming home 1:08.4 behind Kuzmina.
Gossner fared even worse as she failed with seven shots and crossed the finishing line 2:31.0 adrift of the Slovakian in 62nd place to drop to third in the overall rankings.
Norwegian Berger still leads with 601 points with Domracheva now 120 behind in second with German Gossner 20 further back in third.