Jones, a two-time Olympic 100m hurdler switched to bobsleigh after London 2012 and earned a spot on the American team for the opener in Lake Placid.
And the 30-year-old showed she means business as she and Jazmine Fenlator were leading with just one more duo to go.
However, that pair just happened to be Canadian Humphries, who won the world title on the same course earlier this year, and new brakewoman Chelsea Valois and they ended up stealing victory from the Americans as they finished with a total time of 1:54.86 minutes, 0.47 seconds faster than Jones who a little over a month ago finished fourth in the 100m hurdles final at London 2012.
But despite missing out on a debut victory, Jones was delighted with her first World Cup run out.
"I loved it," she said. “I had to rely a lot on Jazmine I am not going to lie but it was fun.
"She was walking me through it as I am so new to it and she kept having to explain everything to me.
"But I think it was a good start and hopefully there is more to come."
Completing the top three in Lake Placid were Americans Elana Meyers and Tianna Madison with a total time of 1:55.34 minutes.