'The Jackal' revealed on Twitter: "I'm upset to have to announce that I have been ruled out of my fight on Saturday by the BBBoC. An old ear injury mildly re-occurred in a spar.
"A specialist initially informed me I was fit to box. Today the BBBoC ruled me out. I'm so so sorry especially to the people who where coming to support me.
"Its been an amazing camp and I'm gutted that I wont be able to showcase my improvements in Wembley."
Frampton's trainer Shane McGuigan tweeted: "Unfortunately today Carl Frampton was overruled by the BBBofC that he cannot box this Saturday due to an old ear injury.
"We were passed fit by another source but today the BBBofC ruled us out. We as a team are bitterly disappointed by this decision.
"We've made some amazing improvements this training camp & will build on everything for a hopeful world title shot soon! Carl is gutted he can't fight for his fans!"
Frampton was due to defend his IBF Intercontinental super bantamweight against Nicaraguan Everth Briceno (35-8-2) - his first fight since leaving Matchroom to be promoted by his manager Barry McGuigan and Frank Warren.