Their first fight saw Abraham win via unanimous decision last summer in Berlin, but Stieglitz ended his reign in only his second defence in emphatic fashion.
Abraham was dominated in three rounds and had his left eye badly swollen by a brutal shot in the second, before the doctor and his cornermen agreed in the fourth that he could not continue.
The win improves Stieglitz’s record to 43-3 and he will hope to improve upon his first spell with the title, which lasted three years and took in six successful defences.
In chief support, Robert Helenius remained undefeated with a one-sided unanimous decision over Michael Sprott.
The British 38-year-old Sprott hardly looked like troubling the Finn, who also saw off Dereck Chisora in 2011.
Helenius is now 19-0 while Sprott has lost 20 of his 56 career fights – more than his latest opponent has even contested.
And George Groves continued to enjoy regular boxing courtesy of new promoters Matchroom via an agreement with Sauerland, with the two camps bringing Carl Froch and Mikkel Kessler back together on May 25.
Groves won his second fight in 15 days by tearing through Baker Bakarat in two rounds via technical knockout.
The 24-year-old Londoner is now 18-0 and will fight again as the semi-main event of Froch-Kessler II at the O2.