The 15-goal striker suffered a broken fibula during the 3-2 win over local rivals Birmingham and will be unavailable for the remaining five matches as Dean Saunders' side look to avoid back-to-back relegations.
Ebanks-Blake, 27, had netted twice at St Andrews before suffering the injury, taking his tally to four goals in his last five games as Wanderers climbed out of the bottom three.
But his brace proved to be his last contribution for the campaign, much to the player's dismay.
He told the club's official website: "Obviously I'm bitterly disappointed to be missing the last five games, but I'll be right behind the lads."
Boss Saunders added: "In the space of 16 days and three games we have lost three players with broken bones.
"Against Bristol City we had Carl Ikeme with a broken hand and Dave Edwards with a foot fracture, and now Sylvan. They are freak injuries and they are ones that we could well do without.
"It was a bitter blow for Sylvan on Monday and for the club too. But it gives someone else a chance."