Bresnan has not played since January 27 - when he took four for 45 in a one-day international against India - after undergoing surgery on a nagging right elbow injury.
Speaking ahead of the three-day friendly, which starts on Tuesday at Headingley, he said: "It has been a while since I played. I'm feeling good and looking forward to playing against Lancashire. I have been bowling in the nets and the elbow has responded well - I feel I'm getting back to my best."
With a hectic international schedule to come in the next 12 months - including back-to-back Ashes series - Bresnan is champing at the bit to return to the England fold.
"For me it is now playing as much cricket as possible with a view to playing well for Yorkshire and ultimately breaking back into the England team," he added.
Batsman Joe Root, who is also looking to play a key role for England this summer and missed the county opener against Sussex, will also feature for Yorkshire in a friendly that will be treated as a training game.
"Both clubs agreed that they wanted to use the next three days as key practice in the middle," said Yorkshire coach Jason Gillespie. "We will use all 13 players at some stage throughout the game and Lancashire will do the same with their squad."