After being asked to bat first, Australia A made 256 for six from their 50 overs, with Callum Ferguson (65) and captain Aaron Finch (57) the top scorers.
Ben Rohrer also made 43 and Tim Paine 38 from 33 balls in Hobart, with Simon Kerrigan comfortably the pick of England's bowlers with figures of two for 27 from his 10 overs.
In reply, James Taylor made an excellent 78 not out from 82 balls, but received precious little support from elsewhere with only Gary Ballance (26) and Toby Roland-Jones (15) reaching double figures.
Ben Foakes, Rikki Clarke and Stuart Meaker were all dismissed without scoring as the Lions slumped to 143 all out in 37 overs, with Alister McDermott taking three for 32 from seven overs.
Things have been no better off the field for the tourists, with Matt Coles and Ben Stokes sent home earlier this week for disciplinary reasons.
Kent seamer Coles and Durham all-rounder Stokes both attended hearings after what an England and Wales Cricket Board statement termed "unprofessional conduct".
Both have apologised for their behaviour.