The Himalayan hill station, which sits almost 1,500 metres above sea level, has never before hosted an international match but was approved to do so on January 27 despite being deep in the winter season. This month has already seen significant snowfall, while weather forecasts suggest the match could be due to start in temperatures at, or just below, freezing point.
The England and Wales Cricket Board have not been told to make any contingencies, but Kohli said: "It's not certain we are going to have a game in Dharamsala. We are all aware (of the weather problems) so this game (Wednesday's fourth ODI at Mohali) becomes all the more important. It makes the game really, really important."
India are 2-1 up with two to play, and Kohli added: "We have to step up tomorrow.
"We will treat the game like it is the last in the series, we have to. But when you lose the first game in a series, that is how you go into every game."
India look likely to field a settled side, after back-to-back victories with the same XI.
Only Ajinkya Rahane is in serious danger of losing his place as opener, with Cheteshwar Pujara waiting in the wings.
But Rahane was man of the match when India defeated England at Mohali in 2011, scoring a career-best 91, and could be given another chance.