The Dutch city is set to host two CSIO5*s - one a dressage leg of the FEI Nations Cup and the other a qualifier for the Furusiyya Nations Cup.
Dujardin, who with Valegro won dressage gold at London 2012, is one of the star attractions and will appear on her stallion for just the second time since her heroics last summer.
During their enforced break - due to the horse's owners' plans to sell him - Dujardin has been riding Uthopia. But a nasty insect bite forced Uthopia out of contention for Rotterdam, leading to a reunion with the as-yet unsold Valegro.
The dressage Nations Cup will be held on Thursday, to leave room on Friday for the show jumping. The highlight of the event will be the dressage Freestyle Grand Prix and, as usual, Sunday afternoon will be punctuated by the Grand Prix jumps.
The German team will be represented in the Furusiyya Nations Cup, after the International Federation ruled they would not be disqualified from the jumping circuit.
There had been fears that Germany's decision not to compete in the severely rain-affected Saint Gallen leg of the tour would lead to their exclusion, given that they had nominated the Swiss venue for points-scoring.
But a committee overruled the regulations and said the withdrawal was justified because of the exceptionally poor conditions and the German's interest in the health of their horses.
Having been given the all-clear, Germany will aim to score points instead in the Netherlands. So too will the hosts, Switzerland and Spain.