Last year's Blue Square Bet Premier winners Fleetwood will welcome two-time European champions Nottingham Forest to Highbury for their first ever Capital One Cup tie.
Micky Mellon's side, who smashed the 100-point barrier on their way to the title, host Forest in the most eye-catching contest of round one which will begin the week commencing August 13.
Wolves start life under manager Stale Solbakken by welcoming Aldershot, who earned a money-spinning home tie with Manchester United in this competition last term, to Molineux.
Beaten finalists Cardiff travel to Northampton while Barnsley boss Keith Hill will go back to Rochdale for the first time since leaving them for Oakwell.
In other stand-out ties, York, who were promoted to npower League Two via the play-offs, face a trip to Doncaster, Paolo Di Canio's Swindon visit Gus Poyet's Brighton while Birmingham, who were winners of the competition two years ago, host Barnet at St Andrew's.
Draw:: Port Vale v Burnley; Derby v Scunthorpe; Rochdale v Barnsley; Notts County v Bradford; Hull v Rotherham; Fleetwood v Nottingham Forest; Carlisle v Accrington; Doncaster v York; Crewe v Hartlepool; Bury v Middlesbrough; Chesterfield v Tranmere; Sheffield United v Burton; Blackpool v Morecambe; Leeds v Shrewsbury;
Preston v Huddersfield; Oldham v Sheffield Wednesday; Cheltenham v Milton Keynes Dons; Ipswich v Bristol Rovers; Stevenage v AFC Wimbledon; Exeter v Crystal Palace; Yeovil v Colchester; Birmingham v Barnet; Bristol City v Gillingham; Northampton v Cardiff; Plymouth v Portsmouth; Wolves v Aldershot; Walsall v Brentford; Millwall v Crawley;
Torquay v Leicester; Dagenham v Coventry; Peterborough v Southend; Swindon v Brighton; Watford v Wycombe; Charlton v Leyton Orient; Oxford v Bournemouth.