Rovers host npower Championship counterparts Millwall in a sixth-round replay on Wednesday night after the sides pulverised each other to a goalless standstill at The Den on Sunday - where Appleton bemoaned "cheap shots" taken by the opposition at his post-match press conference.
"The first 20 minutes or so will probably be very similar," he said. "Both teams probably earning the right to play. Once we get that out of the way we can make sure that we compose ourselves. Hopefully some of the players and footballers we've got in the team can do that."
The Lions' faithful created a fearful din at the weekend as their team came closest to securing victory at the first time of asking.
Blackburn have endured half-empty stands this season as ill-feeling persists among the supporter base over a succession of on and off-field embarrassments witnessed under the Venky's regime.
But with Wembley 90 minutes away and a derby against bitter local rivals Burnley up next in the league, Appleton has called for the kind of vocal backing that could spur his players on.
"We've got to be mindful of the fact that, before I came to the football club, Millwall came here and turned us over 2-0," he said.
"So we'll give them the respect that they deserve but, ultimately, it will be about what we do on the night.
"With us being the home team the onus is on us to get at Millwall and I think the biggest thing, which I hope will be the case on the night, is that we create the atmosphere that Millwall created down at their place.
"If the fans can turn up and make it a memorable night for everyone and cheer every corner, every free-kick, every, throw-in and every tackle then I'm sure it will give the players the edge we might need."