The former Liverpool striker was rested as the npower Championship leaders suffered a shock defeat at struggling Peterborough on Saturday after a busy international break.
The Wales international played 90 minutes for his country against Scotland and then Croatia, two matches which left his hamstrings "screaming", according to manager Malky Mackay.
The Cardiff boss confirmed Bellamy is now fit and raring to go against Rovers as they look to get back to winning ways.
With two games in 48 hours Mackay is likely to shuffle his pack with the likes of Craig Noone, Joe Mason and Craig Conway all pushing for recalls.
Meanwhile, captain Mark Hudson will not play again this season after surgery on an ankle problem this week.