Skipper Jackson and fellow midfielder Hughes were both forced off with knocks during last weekend's 2-1 win over Wolves.
Boss Chris Powell revealed the duo were suffering with fatigue as much as anything, so they could be available.
Midfielder Dale Stephens and striker Danny Haynes have returned to training after their recent lay-offs and could come into the reckoning, but defender Leon Cort remains a doubt with a knee problem.
Tottenham loanee Jon Obika is pushing for a start after climbing off the bench to score the last-minute winner against Wolves.
The Addicks still have a slim chance of reaching the play-offs but need a win and other results to go their way to take it to the final week.