Striker Holmes has been ruled out for the rest of the season following the recurrence of a foot injury he suffered two seasons ago.
Meanwhile, Iro and Sekajja have returned to Stevenage and Crystal Palace respectively following the end of loan spells with the Bees.
New signing Craig Beattie, who joined the club last week, is now eligible to play for Davids' team and is expected to play some part.
Midfielder Curtis Weston featured for the last 10 minutes of Barnet's 0-0 draw with Port Vale last weekend on his return from illness and could be back in the starting line-up.
Davids will also be without goalkeeper Liam O'Brien who faces a lengthy spell on the sidelines with a groin injury.