The world and European champions return to action for the first time since losing the Confederations Cup final 3-0 to Brazil on June 30.
They do so with regulars such as Gerard Pique, Xavi and Sergio Busquets rested and replaced by fringe players and members of Spain's Under-21 side, who were crowned European champions in Israel in June.
The squad was released through the team's official Twitter feed @sefutbol.
Spain are on course to qualify for the World Cup finals in Brazil next year as they top Group I with 11 points from five games, a point ahead of France, with three left to play.
Their next qualifier is away to third-placed Finland in Helsinki on Sept 6.
Forwards: Jesus Navas (Manchester City), David Silva (Manchester City), Cesc Fabregas (Barcelona), Alvaro Negredo (Manchester City), Fernando Llorente (Juventus), Cristian Tello (Barcelona)