Gayle, who has scored seven goals in nine appearances for Posh since arriving on loan from Dagenham & Redbridge, was cup-tied for the defeat to Norwich last weekend.
Boss Darren Ferguson will also see his squad swelled by the addition of Manchester United pair Scott Wootton and Davide Petrucci.
Defender Wootton has returned until the end of the season having enjoyed a previous loan spell at London Road.
He was kept out of the side by Ryan Bennett and Gabriel Zakuani last time round but Zakuani's involvement in the African Nations Cup is likely to give him his chance.
Midfielder Petrucci has been drafted in on a one-month loan deal.
Forward Lee Tomlin will serve the second game of a three-match suspension while Peter Grant and Kgosi Ntlhe remain long-term absentees.