Centre-half Hartley has been struggling with a knee injury this week, but manager John Hughes has no other new injury concerns.
Pools, relegated in midweek following Oldham's win over Yeovil, are chasing a third straight league win.
Midfielder Antony Sweeney and defender Evan Horwood, named on the bench at Tranmere last week following respective hamstring injuries, are both pushing for recalls.
Teenage defender Jordan Richards remains sidelined with a knee injury, while goalkeeper Andy Rafferty continues to build up his match fitness following knee surgery last summer which has kept him out all season.
Pools have lost only one of their last nine league games at Victoria Park.