The two sides meet in a repeat of the 1985 FA Cup quarter-final clash which was marred by crowd problems and hooliganism and led to a blanket ban on away supporters by Luton which lasted for four years.
Both Millwall and Luton beat top-flight opposition to set up the tie, with the Lions overcoming Aston Villa and Luton causing an upset against Norwich.
Despite facing a side three divisions below them in the league pyramid, Jackett is expecting a close game at Kenilworth Road.
"We won't be underestimating Luton in any way, shape or form when you see them beating the calibre of Norwich and Wolves this season," he told Press Association Sport.
"It (Norwich) was a great win, I've watched the game and Luton went to Carrow Road and they played very, very well on the day.
"It was a fantastic performance, they got their goal when they needed to and defended well."