Poole sustained a knock to his leg in the recent defeat at Oldham and missed last Saturday's home draw with Walsall, while Middlesbrough loanee Wyke has been struggling with a heel problem that has restricted him to bench duty in the last two matches.
Manager John Hughes was given a boost this week as Jack Baldwin completed 45 minutes for the reserves.
The teenage defender had been expected to miss the rest of the season after damaging medial knee ligaments earlier this month.
Another teenager, striker Luke James, staked his claim for a recall this week with a hat-trick for the reserves.
Bottom club Pools are chasing their first win in seven as they bid to keep their survival hopes alive.