Best scored for Rovers' reserves earlier this week as he continued his rehabilitation from cruciate knee ligament damage sustained during pre-season but, as is common when returning from such injuries, the Republic of Ireland international is suffering from swelling around the joint.
"If Leon is physically okay and his knee is okay then I'd like to get him involved if I can," said boss Michael Appleton on Blackburn's official website.
"But every now and again his knee does get a little bit of swelling after certain training sessions, so again it will come down to the player.
"It is pretty much the norm, but the timing isn't great."
Fabio Nunes also featured for the second string following a hamstring injury lay-off and could come into contention, although fellow winger Josh King is a fortnight away due to a similar complaint.
Lee Williamson, Colin Kazim-Richards (both ankle), Martin Olsson (eye), Dickson Etuhu (knee), David Dunn (calf) and Paul Robinson (back) remain sidelined.