Attacking player Murphy, 23, completed his permanent move from Motherwell for an undisclosed fee on Thursday and could make his debut for the club.
Defender Harry Maguire is also available again after sitting out the New Year's Day draw at Doncaster through suspension.
Wilson, who has no new injury or suspension worries, is likely to retain recent signing Danny Higginbotham in his back four following Neill Collins' cheekbone fracture, while teenagers Terry Kennedy and Elliott Whitehouse, who both made their debuts on the New Year's Day, and another 19-year-old, Joe Ironside, are hoping to keep their places in the side.
Goalkeeper Mark Howard (thigh) returned to training this week, but is hoping to feature in a couple of development games before being considered for his long-awaited first-team return.
The Blades beat Oxford 3-0 at Bramall Lane in the first round of last year's competition.