Osbourne missed Saturday's 4-2 defeat at Middlesbrough as he served a one-match ban for collecting five yellow cards this season.
On-loan Aston Villa forward Nathan Delfouneso made way for midfielder Angel Martinez before half-time at the Riverside Stadium but, with Pool 2-0 down at that stage, boss Michael Appleton's decision appeared to be a tactical one.
Having slammed their first-half display at Boro as the worst during his time in charge, Appleton is expecting a response against the Tigers.
"It's probably the best game we could have," he told Blackpool's official website. "It's in front of our own fans and it's obviously against a team that are flying. Hopefully we react properly and in the right way to it."
Defender Ian Evatt (knee) and winger Matt Phillips (toe) remain on the sidelines.