Striker Paynter will serve the second game of a three-match suspension for the red card he received during the 2-2 home draw with Sheffield United on New Year's Day.
Dean Saunders left to take over at Wolves this week so former Wales Under-21 boss Brian Flynn, who has been working for Rovers as a scout, and captain Rob Jones have been placed in temporary charge of first-team affairs while the South Yorkshire club conduct their search for a new manager.
Flynn and Jones could have Martin Woods back from the thigh injury that has kept him out since November, while fellow midfielder James Coppinger is hoping to make his 300th league appearance for Doncaster.
Another milestone is within reach on Saturday. Rovers can break a club-record of 11 consecutive unbeaten away matches if they avoid defeat at the Lamex Stadium.