Mousinho has endured an injury-ravaged first season at Deepdale and last played in January's 1-0 FA Cup third-round defeat against Millwall.
But the former Stevenage midfielder now has a week's full-training under his belt following a hamstring problem.
Stuart Beavon is expected to continue in attack after returning from injury to score during last weekend's 2-0 win over Bournemouth - North End's first victory for six league games.
Joel Byrom is facing an absence of between four to six weeks after undergoing hernia surgery and the midfielder joins defender Scott Laird (broken leg) among the long-term absentees.
