Hughes missed training last week with a sore back and he took a hefty knock during the 2-1 defeat at Brighton.
Manager Nigel Clough is keeping his fingers crossed that the 17-year-old's injury settles in the next few days with Forest due at Pride Park for a lunchtime kick-off on Saturday.
"He is extremely uncomfortable, it's very sore," said Clough following his side's loss to Albion. "He got a knee in the back from an aerial challenge - that was another one (foul) the referee missed.
"Will doesn't usually stay down. He gets whacks and gets straight back up again, so when he does stay down you get a little bit concerned about him."
Meanwhile, Craig Bryson was absent for a fourth consecutive game at Brighton on Saturday due to a knee injury and the midfielder remains a doubt for the Forest fixture.
Clough added: "He certainly couldn't have played at Brighton. He's got a chance for the weekend but, ideally, it's a two or three-week job.
"We'll get him moving towards the middle of this week and see if his knee has settled down."