The Albion boss saw his side go down to Chesterfield, who ended the Brewers' club-record nine-match victorious home streak courtesy of Drew Talbot's superb 52nd-minute strike.
A philosophic Rowett said: "That defeat was always going to come at some point and the key thing now is to deal with it well and get back on the horse.
"These things happen and it's about what you do in the next game. It doesn't really change anything because we knew we would still have to win more games - and we'll go to Accrington on Monday and Port Vale on Friday with that mindset."
Burton struggled to find their usual attacking tempo in what could be best described as a lacklustre first half.
Top scorer Jacques Maghoma skied a shot from the edge of the area while Ian Sharps and Robbie Weir both failed to hit the target with headers. Chesterfield took the lead when Talbot found the space to beat Stuart Tomlinson with a stunning right-foot shot from 30 yards out.
Rowett said: "It was disappointing in the sense that we didn't play the way we usually do, but I have to say first and foremost that Chesterfield are a good side and showed a lot of quality in the game.
"We just couldn't find our usual energy, tempo and the forward running that has put is where we are in the league table. At the same time, that's 10 home games and we've won nine of them which is still a great achievement."
The victory lifted Chesterfield to 10th in the table and kept alive their hopes of a late charge for a place in the play-offs. Manager Paul Cook said: "We've got a great game to look forward to now at home to Port Vale on Monday and we've got to keep fighting to see if we can make the play-offs.
"I'm proud of the performance here but I'm not proud of our league position - and that's something we'll be trying to improve between now and the end of the season."