On-loan midfielder Rose was ineligible for Saturday's 2-0 victory over parent club Fleetwood, Scott's first game at the helm having replaced sacked predecessor Dean Holdsworth just 24 hours earlier.
Left-back Tonkin, meanwhile, served the second game of his two-match suspension, yet both return to contention at the Proact Stadium as the Hampshire club go in search of three successive league wins for the first time since March last year.
Scott will also assess Lionel Ainsworth, Troy Brown and Jeff Goulding to see whether any of the injured trio are fit enough to be welcomed back.
A couple of unknown players are also carrying the usual minor bumps and bruises after the weekend and will be monitored.
