Veteran striker Hughes had a virus last weekend while fellow forward Tomlin also missed the home win over Coventry with a groin problem. However, both players have resumed training to compete with Ben Williamson and Louis Dodds for a starting place alongside Tom Pope.
Daniel Jones is also back in training after missing the last four matches with a groin strain but Saturday looks like coming too soon for the left-back. Rob Taylor has a calf strain and will probably not be involved.
First-team coach Rob Page will be in charge at Prenton Park with manager Micky Adams recovering from the hip operation he underwent on Monday.