Vaughan received his 10th booking of the season in Saturday's 4-1 defeat at Brighton and will sit out the first of a two-match ban.
Lee Novak and Alan Lee will be vying for a place up front as Jermaine Beckford remains sidelined with a hamstring injury.
Manager Mark Robins could also be without defender Anthony Gerrard, who was forced off in the closing stages at the weekend with a recurrence of his on-going Achilles problem.
Joel Lynch replaced Gerrard and could continue if he misses out again, but Murray Wallace will also come into contention.
Right-back Calum Woods will return to the squad after missing out against Brighton with a hamstring strain.
Huddersfield, five points above the relegation zone, have won only one of their last six league matches.