The on-loan defender serves the second game of a three-match ban following his red card against Morecambe.
Defender Terrell Forbes, striker Scott Boden and winger Jack Waddle have all been placed on the transfer list and are not expected to be involved.
Cook has no fresh injury concerns for the fixture but is expected to make changes following the 1-0 defeat at Accrington on New Year's Day.
Jack Lester and Marc Richards were paired in a front two at the Crown Ground but Cook could revert to playing one striker while midfielder Dan Whitaker may be recalled to the starting XI.
The Spireites have won just one of their last five matches and are four points off the play-off places.