The Seagulls were last in action on December 18 and the prolonged break has allowed Gary Dicker, Andrea Orlandi and Bruno to return to training.
Full-back Bruno has missed the last two games with an Achilles injury, while midfielder Dicker has been out for almost a month with a knee problem.
Spanish midfielder Orlandi has recovered from a rib injury but could be restricted to a role on the bench.
The fixture will see Poyet go head-to-head with former Chelsea team-mate Gianfranco Zola and the Uruguayan is looking forward to the challenge.
"It's good to see him doing well, I'm not jealous at all, I just want to see him doing well," Poyet told the club's official website.
"If I cannot win the league, the person I want to win the league is Gianfranco Zola."