Former under-23 world champion Duarte was second on stage five of the race last year while his 23-year-old Colombian compatriot Atapuma makes his Grand Tour debut but has some pedigree, having won a stage of the Giro del Trentino last year.
Jarlinson Pantano and Robinson Chalapud will also look to shine in the mountains as well.
Carlos Julian Quintero, Wilson Marentes and Dalivier Ospina are also in the nine-man team and rounding out the line-up is veteran Leonardo Duque and youngster Edwin Avila.
"We tried to set-up a balanced group, capable of staying in the action in every race situation," general manager Claudio Corti stated.
"Even if our main focus will be on the mountains, where our top athletes can show their best qualities. We are going to the Giro to show off and put up a big show, that is what everyone is expecting from us.”
The event starts in Naples on Saturday and is exclusively live in the UK on British Eurosport.