The 31-year-old had asked to leave Carrow Road less than a week after the club's first season back in the Premier League ended in a comfortable mid-table finish, and that the Canaries turned his transfer request down.
But Holt has now insisted on his Twitter account that he is looking to leave the club as soon as possible after disagreements with the board.
"I have loved my three years at the club and it saddens me to write this but I want you to here it from me first," Holt wrote on the social networking site.
"Just to stop the rumour mill again. I have expressed to the board and the CEO that I want to leave the football club. Due to disagreements."
Journeyman striker Holt made a big impact on the Premier League in his first season in the top flight, scoring 15 goals in the competition as Paul Lambert's team defied expectations by easily averting a relegation battle. That haul made him the second-highest English goal scorer of the season, behind only Wayne Rooney, and saw him win the club's the player of the year award for the third successive season.
There had been speculation that the big centre-forward, whose former clubs include Shrewsbury Town, Nottingham Forest and Rochdale, might be a surprise call-up to England for this summer's European Championships.
However, new England manager Roy Hodgson did not select him, instead picking Danny Welbeck, Andy Carroll and Jermain Defoe as well as Rooney.