The two players were unable to feature in Saturday's 1-0 win over Norway due to fitness problems but manager Roy Hodgson will count on both men in Poland and Ukraine.
Johnson is expected to start the tournament as England's first-choice right-back while Danny Welbeck will compete with Andy Carroll and Jermain Defoe for a place up front with Wayne Rooney suspended for the opening two games.
There were no surprises in the England squad, with Phil Jagielka formally confirmed as the replacement for Gareth Barry, who was forced out with a torn muscle in his lower abdomen on Monday.
England were also forced to replace third-choice goalkeeper John Ruddy with Birmingham City's Jack Butland prior to the Norway victory after the Norwich star broke a finger in training.
Hodgson has confirmed the squad numbers England will wear at the Euro 2012 finals:
1 Joe Hart
2 Glen Johnson
3 Ashley Cole
4 Steven Gerrard
5 Gary Cahill
6 John Terry
7 Theo Walcott
8 Frank Lampard
9 Andy Carroll
10 Wayne Rooney
11 Ashley Young
12 Leighton Baines
13 Rob Green
14 Phil Jones
15 Joleon Lescott
16 James Milner
17 Scott Parker
18 Phil Jagielka
19 Stewart Downing
20 Alex Oxlade-Chamberlain
21 Jermain Defoe
22 Danny Welbeck
23 Jack Butland
