The 52-year-old's international quad wheelchair tennis career has spanned 12 years and seen him collect 16 Grand Slam titles along with his two Games golds.
But having only managed quad doubles silver at London 2012, Norfolk is now choosing to concentrate on growing his business, Equipment for the Physically Challenged, as well as spending more time with his wife Linda and two young children.
However Norfolk may still compete at some international tournaments, including the British Open Wheelchair Tennis Championships in Nottingham in July.
"I haven't had a break from the Tour for 12 years and I feel it is time to dedicate some of my time to other areas of my life," he said. "I have enjoyed some incredible moments in my career and I want to thank everyone who has been a part of that, especially those who have given up their time and efforts to help me get to the top.
"I look forward to staying involved with the Tennis Foundation and I hope I can inspire more disabled people in this country to take up tennis, as it's such a brilliant and fun sport to play."