Eurosport - England U21's Connor Wickham scores (PA Photos)
Stuart Pearce's Young Lions will take on Serbia at the home of Norwich on Friday, October 12 (kick-off 6pm), shortly before the senior side host San Marino in their latest World Cup qualifier at Wembley.
Details of the Under-21s' second leg have still to be announced, although it will almost certainly take place four days later.
The play-off sees England attempt to secure a fourth straight finals appearance after they won their qualifying group.
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