Rowsell joined forces with Dani King and Laura Trott at London 2012 to claim team pursuit gold, adding to the world title the trio picked up en route to the capital.
That Track Cycling World Championships gold was Great Britain's fourth in five years but if they are to make it five out of six then they will have to do so without Rowsell after she opted to focus on the road this year.
The women's team pursuit is changing from three riders and three kilometres to four riders and four kilometres after this season, and 24-year-old Rowsell is expected to slip back in for the 2014 season and the Rio Olympics.
But in the meantime her place at next month's World Championships in Minsk is likely to go to either Elinor Barker, who rode in Rowsell's place in November's Glasgow Track World Cup, or Amy Roberts.
And Rowsell is adamant that whoever gets the nod to ride with King and Trott will do the business.
"I'm fully confident they're going to win," three-time world champion Rowsell said. "They won the Glasgow World Cup with very limited preparation.
"Seeing Elinor ride there made me think 'it's okay, they're going to be fine'.
"Elinor's a fantastic talent and also Amy Roberts. Riders like Elinor and Amy need that sort of experience because next year we're going to need four riders anyway."