The Ospreys will begin the defence of their RaboDirect Pro12 title with a trip to Italy to face Treviso.
The Welsh region's thrilling victory over European champions Leinster in last season's final made them the first side to secure four titles.
The season begins on the weekend of September 1, and Steve Tandy's side will travel in the knowledge that they have never lost to Treviso, although they were held to a draw in the Heineken Cup at the Stadio Comunale di Monigo last season.
Leinster begin their season with a testing trip to face Simon Easterby's Scarlets, while new Munster coach Rob Penney will lead the province for the first time when they face Edinburgh at Murrayfield.
Cardiff Blues director of rugby Phil Davies begins his time in charge with a meeting against Connacht in Galway.
Glasgow Warriors, with Gregor Townsend at the helm, open with a visit to Ravenhill to meet beaten Heineken Cup finalists Ulster and their new coach Mark Anscombe.
Italian side Zebre, who have taken the place of the now defunct Aironi, go to Rodney Parade to face the Newport Gwent Dragons.
Glasgow will play their first game at their new Scotstoun Stadium home against the Scarlets the following weekend, with Zebre hosting Connacht in their opening fixture on their own turf.
Elsewhere on the second weekend the Blues meet Edinburgh as they return to Cardiff Arms Park, Ulster travel to the Ospreys, Leinster host the Dragons and Munster clash with Treviso.
The Christmas and New Year period is again set aside for local derbies, while the semi-finals will take place over the weekend of May 11, with the final to be held on Saturday, May 25.