Mathers had a bottle and a cup of coffee thrown at him after he scored one of Wakefield's tries during their 37-16 victory at the Wish Communications Stadium. The Tigers are investigating the incident and say anyone found guilty of throwing missiles will be banned.
The club's interim chief executive Steve Gill said: "We are taking a zero-tolerance approach to this kind of behaviour and obtaining evidence through CCTV footage and the assistance of stewards to identify the people involved and pass on to the authorities."
He added: "The club has a family reputation that it has held for many years and recognise that the vast majority of loyal fans are let down by the very small minority who act in a disrespectful manner on rare occasions.
"These individuals cannot be allowed to drag the club through the gutter."