Myler, Luther Burrell, Courtney Lawes, Lee Dickson, Ben Foden and England captain Tom Wood all tasted bitter defeat to arch-rivals Leicester in Saturday's Aviva Premiership final. England play a combined South American team next Sunday before tackling Argentina in two Test matches.
"My job is to pick them up, give them a clear target going into next Sunday and get some of them motivated to play again," Lancaster said.
"The likes of Courtney Lawes, Tom Wood, Lee Dickson, I thought they put in an unbelievable shift and I want to make sure they come back to England and get back on the horse.
"I want to make sure everyone has an opportunity to play but I will prioritise the Tests. Stephen will probably get a go next week and then Freddie (Burns) will be back in the equation."
Burns scored 20 points, including a try after 39 seconds, as he inspired England to a 40-12 victory over the Barbarians at Twickenham on Sunday. With Owen Farrell and Toby Flood absent, the creative Gloucester fly-half travels to south America tonight as first-choice fly-half.
Myler was called into the England squad as a replacement for Alex Goode and he may need to be picked up by Lancaster more than most after his gaffe at the end of the first half led indirectly to Northampton captain Dylan Hartley being sent off.
Had he followed instructions from referee Wayne Barnes and grubbered his 22 drop-out into touch then the half-time whistle would have blown. But Myler kicked the ball straight out. Leicester opted for a scrum and Barnes awarded them a penalty, a decision which appeared to prompt Hartley's outburst.
Despite being down to 14 men, Northampton battled valiantly with Foden back to his searing best form while Burrell gave the Saints power and go-forward in the midfield.
"I was really pleased for Ben. He has come back into form too late for the Lions but he was exceptional," Lancaster said.