Pool Two has come down to a decider between two clubs with six European titles between them.
Toulouse are currently two points above their hosts, but they were beaten twice by English opposition in last season's tournament and Leicester have not lost a Heineken Cup game on home soil since Munster toppled them more than six years ago.
"We will come flying out of the blocks, and it is always easier to come out of the blocks when you are at home," Tigers' England scrum-half Ben Youngs said.
"These are the games that you want to play in as a professional. It is not very often that you get the chance to play against a side like Toulouse.
"They are a world-class side that has won the Heineken Cup and the Top 14 countless times. To have them come to Welford Road and be able to have a crack at them is exciting.
"We need to start well and play from the first whistle. We need to control the ball a bit better in their 22 as well.
"We turned it over a couple of times at the Ospreys last weekend, and we need to be more clinical and tidy up a few things."
Leicester rugby director Richard Cockerill has made a solitary change to the team held 15-15 by the Ospreys in Swansea last Sunday, with number eight Thomas Waldrom replacing Jordan Crane.
Toulouse field their familiar galaxy of international talent, their formidable strength being underlined by a replacements' bench that features Maxime Medard, Yannick Jauzion, Luke Burgess and Yannick Nyanga.