The 31-year-old is one of six of the world’s top 10 female players who will take to the courts at Devonshire Park for the tournament from June 15-22 as the game’s elite begin their final preparations for SW19.
Li, who lost in the final of the Australian Open in January, has twice made the quarter-finals at Wimbledon in her career and the Chinese player is hoping for even better this year.
She said: “I am delighted to be heading back to the Aegon International after having such a good time when I was last in Eastbourne in 2011.
“It is great preparation for Wimbledon as the grass courts are immaculate, and the fans there are so knowledgeable about tennis.”
The 2011 French Open winner joins Agnieszka Radwańska, Sara Errani, Petra Kvitova, Caroline Wozniacki and Angelique Kerber in the field.
Britain’s Laura Robson and Heather Watson will be leading home hopes while last year’s champion Tamira Paszek will be returning to defend her title.
The men’s draw is headed by Juan Monaco, Gilles Simon and Philipp Kohlschreiber.