Lendl, who began coaching Murray last year, was invited by British player Ross Hutchins to play in the June event 16 at The Queen's Club.
The event will raise money for The Royal Marsden Cancer Charity, which supports The Royal Marsden - the world-leading cancer centre treating Hutchins, who was diagnosed with Hodgkins Lymphoma last December.
'I am delighted to be playing in the Rally Against Cancer event on the brilliant centre court at The Queen’s Club,” said Lendl, who twice won the titles at The Queen's Club, in 1989 and 1990. “
The event is for a great cause - the Royal Marsden Cancer Charity - and I'm very excited to be playing on a grass court for the first time in 20 years.
"To be able to play with Andy, Tim and Tomas will be a joy for me and I look forward to the match. I have got to know Ross well over the past couple of years and have known his family for a long time. I am right behind him and everyone in similar situations to him."