Bruyneel was named in the United States Anti-Doping Agency report released this week which said he was one of the people who had helped Armstrong organise doping within the U.S. Postal Team.
"In light of these testimonies, both parties feel it is necessary to make this decision since Johan Bruyneel can no longer direct the team in an efficient and comfortable way," RadioShack Nissan said in a statement.
Belgian Bruyneel was Armstrong's team manager when the Texan won his seven Tours from 1999-2005 as well as during his two Tour rides in 2009 and 2010 after his comeback from a 3-1/2 year retirement.
- Johan Bruyneel