Cole will be replaced by his young understudy Ryan Bertrand who played on the left side of midfield in the penalty shootout win over Bayern Munich in last season's final.
"Ashley Cole won't be available tomorrow, he has a problem with his hamstring, shouldn't be out for long," coach Roberto Di Matteo told reporters on Tuesday.
"Ryan Bertrand has always performed well for us when he's played and I have full confidence he will play a great game."
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