Best, who suffered cruciate knee ligament damage shortly after a £3million summer move from Newcastle, made his long-awaited Rovers debut from the bench during the closing stages of Tuesday's night's 1-0 npower Championship defeat at Bolton.
The Republic of Ireland international appears set to partner Jordan Rhodes in attack as DJ Campbell is cup-tied.
Campbell's fellow loanees Cameron Stewart and David Jones are also unavailable, meaning Josh King and David Dunn could be named among the substitutes despite only returning to training on Friday from respective hamstring and calf problems.
Colin Kazim-Richards, Lee Williamson (both ankle), Martin Olsson (eye), Dickson Etuhu (knee) and Paul Robinson (back) remain unavailable.
- Sports & Recreation
- Jordan Rhodes