With first-choice keeper David Martin set to be absent for a fifth game due to a back complaint, the Dons need McLoughlin to be fit or manager Karl Robinson will have to field an academy teenager on Tuesday night.
McLoughlin was nursing an ankle injury following the goalless draw with Sheffield United on Saturday but the Republic of Ireland Under-21 international is expected to be okay.
Daniel Powell is a doubt after the winger pulled up after half-an-hour at Bramall Lane.
On-loan striker Patrick Bamford made his return from over two months out with a hamstring strain at the weekend and the Chelsea teenager will be involved again after suffering no ill effects.
Midfielder Stephen Gleeson got another 15 minutes under his belt against the Blades as he continues his rehabilitation from a broken foot and the playmaker is expected to be named on the bench again.
However, winger Luke Chadwick will remain out with a hip flexor problem.